A simple PCR-based fluorometric system for detection of mutant fusion DNAs using a quencher-free fluorescent DNA probe and graphene oxide.
نویسندگان
چکیده
We propose a facile fluorometric system for detection of gene mutations using graphene oxide (GO). A fluorescent probe DNA anneals to a specific mutant gene and is degraded by the 5'→ 3' exonuclease activity of Taq polymerase during PCR, and the released fluorophore retains fluorescence after addition of GO without quenching.
منابع مشابه
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ورودعنوان ژورنال:
- Chemical communications
دوره 51 32 شماره
صفحات -
تاریخ انتشار 2015